Construction of a Bacterial Cellulase Cocktail for Saccharification of Regenerated Cellulose and Pretreated Corn Stover
نویسندگان
چکیده
منابع مشابه
Construction of a Bacterial Cellulase Cocktail for Saccharification of Regenerated Cellulose and Pretreated Corn Stover
To apply bacterial cellulases for efficient saccharification of biomass, three Clostridium thermocellum cellulases and a Thermoanaerobacter brockii β1,4-glucosidase were synthesized in Escherichia coli, and the proportions among them were optimized. When the activities of CelD, CBHA, CBH48Y, and CglT were set at 554, 0.91, 0.91, and 856 mU per assay, respectively, the percent conversion of rege...
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BACKGROUND Simultaneous saccharification and co-fermentation (SSCF) process involves enzymatic hydrolysis of pretreated lignocellulosic biomass and fermentation of glucose and xylose in one bioreactor. The optimal temperatures for enzymatic hydrolysis are higher than the standard fermentation temperature of ethanologenic Saccharomyces cerevisiae. Moreover, degradation products resulting from bi...
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As a promising alternative of fossil fuel, ethanol has been widely used. In recent years, much attention has been devoted to bioethanol production from lignocellulosic biomass. In previous research, it is found that the pretreatment method named low-moisture anhydrous ammonia (LMAA) has the advantage of high conversion efficiency and less washing requirements. The purpose of this study was to e...
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UNLABELLED BACKGROUND Previous research on alkaline pretreatment has mainly focused on optimization of the process parameters to improve substrate digestibility. To achieve satisfactory sugar yield, extremely high chemical loading and enzyme dosages were typically used. Relatively little attention has been paid to reduction of chemical consumption and process waste management, which has prov...
متن کاملBSA treatment to enhance enzymatic hydrolysis of cellulose in lignin containing substrates.
Cellulase and bovine serum albumin (BSA) were added to Avicel cellulose and solids containing 56% cellulose and 28% lignin from dilute sulfuric acid pretreatment of corn stover. Little BSA was adsorbed on Avicel cellulose, while pretreated corn stover solids adsorbed considerable amounts of this protein. On the other hand, cellulase was highly adsorbed on both substrates. Adding a 1% concentrat...
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ژورنال
عنوان ژورنال: BioResources
سال: 2015
ISSN: 1930-2126
DOI: 10.15376/biores.10.4.7681-7692